What is a hpv dna amp probe

what is a hpv dna amp probe

Human Papilloma Virus (HPV) FAQ

Sep 25,  · The HPV DNA test is used to check for high-risk HPV infection in women. HPV infection around the genitals is common. It can be spread during sex. Some types of HPV can cause cervical cancer and other cancers. These are called high-risk types. Low-risk types of HPV may cause genital warts in the vagina, cervix, and on the skin. The virus that causes warts can be spread when . The cobas HPV test detects DNA of the high-risk types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and This test does not detect DNA of HPV low-risk types (eg, 6, 11, 42, 43, 44) since these are not associated with cervical cancer and its precursor lesions. The cobas HPV test is not recommended for evaluation of suspected sexual abuse.

The most important risk factor for developing cervical cancer is infection with human papillomavirus HPV. Doctors can test for the high-risk HPV types that are most likely to cause cervical cancer by looking for pieces of their DNA in cervical cells.

The test can be done by itself primary HPV test or at the same time as a Pap test called a co-test. Some HPV tests are approved only as part of a co-testwhen the HPV test and the Pap test are done at the same time to screen for cervical cancer.

Because a primary HPV test may not be an option everywhere, a co-test every 5 years or a Pap test every 3 years are still good options. All the screening tests primary HPV test, co-test, and Pap test are good at finding cancer and pre-cancer.

The primary HPV test is better at preventing cervical cancers than a Pap test done alone and does not add more unnecessary tests, which can happen with a co-test. The most important thing to remember is to get screened regularly, no matter which test you get. The result of the HPV test, along with your past test results, determines your risk of developing cervical cancer. If the test is positive, this could mean more follow-up visits, more tests how to get a handicap parking permit in illinois look for a pre-cancer or cancer, and sometimes a what is a hpv dna amp probe to treat any pre-cancers that might be found.

Because there are many different follow-up or treatment options depending on your specific risk of developing cervical cancer, it is best to talk to your healthcare provider about your screening results in more detail, to fully understand your risk of cervical cancer and what follow-up plan is best for you.

Definition

The HPV DNA test is used to check for high-risk HPV infection in women. HPV infection around the genitals is common. It can be spread during sex. Some types of HPV can cause cervical cancer and other cancers. These are called high-risk types. Low-risk types of HPV may cause genital warts in the vagina, cervix, and on the skin. The virus that causes warts can be spread when you have sex. HPV DNA is the preferred approach when liquid-based cytopathology is used for screening. All women who test positive for high-risk HPV DNA should be referred for colposcopic evaluation. Women with ASC-US who test negative for high-risk HPV DNA can be followed up with repeat cytologic testing at 12 months ( ASCCP Guidelines - JAMA ; ). Nov 01,  · Doctors can test for the high-risk HPV types that are most likely to cause cervical cancer by looking for pieces of their DNA in cervical cells. The test can be done by itself (primary HPV test) or at the same time as a Pap test (called a co-test). You won’t notice a difference in .

Persistent infection with human papillomavirus HPV is the principal cause of cervical cancer and its precursor cervical intraepithelial neoplasia CIN. HPV is a small, nonenveloped, double-stranded DNA virus, with a genome of approximately 8, nucleotides. There are more than different types of HPV and approximately 40 different HPVs that can infect the human anogenital mucosa.

However, data suggest that 14 of these types HPV types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68 are considered high risk HR for the development of cervical cancer and its precursor lesions.

Furthermore, HPV types 16 and 18 have been regarded as the genotypes most closely associated with progression to cervical cancer. Although persistent infection with HR HPV is necessary for the development of cervical cancer and its precursor lesions, only a very small percentage of infections progress to these disease states. However, almost all infected women will mount an effective immune response and clear the infection within 2 years without any long-term health consequences.

In developed countries with cervical cancer screening programs, the Pap smear has been used since the mids as the primary tool to detect early precursors to cervical cancer.

Although it has decreased the death rates due to cervical cancer dramatically in those countries, the Pap smear and subsequent liquid-based cytology methods require subjective interpretation by highly trained cytopathologists and misinterpretation can occur.

Cytological abnormalities are primarily due to infection with HPV; however, various inflammatory conditions or sampling variations can result in false-positive cytology results. Triage of an abnormal cytology result may involve repeat testing, colposcopy, and biopsy. A histologically confirmed high-grade lesion must be surgically removed or ablated in order to prevent the development of invasive cervical cancer.

Proper implementation of nucleic acid testing for HPV may 1 increase the sensitivity of cervical cancer screening programs by detecting high-risk lesions earlier in women 30 years and older with normal cytology and 2 reduce the need for unnecessary colposcopy and treatment in patients 21 and older with cytology results showing atypical squamous cells of undetermined significance ASC-US.

Recently, data suggest that individual genotyping for HPV types 16 and 18 can assist in determining appropriate follow-up testing and triaging women at risk for progression to cervical cancer. Negative for human papillomavirus HPV genotypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and A positive result indicates the presence of human papillomavirus HPV DNA due to 1 or more of the following genotypes: 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and This test does not detect DNA of HPV low-risk types eg, 6, 11, 42, 43, 44 since these are not associated with cervical cancer and its precursor lesions.

Prevalence of HPV infection in a population may affect performance. Positive-predictive values decrease when testing populations with low prevalence or individuals with no risk of infection. Cervical specimens often show visibly detectable levels of whole blood as a pink or light brown coloration. These specimens are processed normally on the cobas System.

If concentrations of whole blood exceed 1. The cobas HPV Test performance has not been validated with PreservCyt specimens that have been treated with glacial acetic acid for removal of red blood cells. The cobas HPV Test performance has not been validated with PreservCyt specimens that have been filled past the maximum fill line of the primary vial. ThinPrep vials that have had any additional PreservCyt fluid volume added or any dissimilar fluid volume added to the initial specimen should not be submitted for testing.

The cobas HPV Test is not intended for use in determining the need for treatment ie, excisional or ablative treatment of the cervix in the absence of high-grade cervical dysplasia. The cobas HPV test is not intended for use with samples other than those collected by a clinician using an endocervical brush or spatula and placed in the ThinPrep Pap test PreservCyt solution.

HPV-negative cancers of the cervix do occur in rare circumstances. Use of this device for primary cervical cancer screening should be undertaken after carefully considering the performance characteristics put forth in the cobas HPV Test label, as well as recommendations of professional guidelines.

The effects of other potential variables such as vaginal discharge, use of tampons, douching, etc, and specimen collection variables have not been evaluated.

Table 1. The results are summarized below in Table 2. Table 2. Comparison of the Roche cobas HPV test to cervical biopsy among samples demonstrating atypical squamous cells of undetermined significance ASC-US or worse by cytology Pap smear.

In comparison, the current Digene hc2 assay demonstrated a sensitivity of Table 3. J Low Genit Tract Dis ;16 3 J Pathol ; Lancet Oncol ; Am J Clin Pathol Oct; 4 Skip to main content. Register Sign In. Test Catalog Account. Outreach Solutions Tactics Articles Events.

Utilization Management Resource Center Algorithms. Test Catalog. Download Test. The cobas HPV test is not recommended for evaluation of suspected sexual abuse. The presence of PCR inhibitors may cause false negative or invalid results. The cobas HPV Test is not intended for women who have undergone hysterectomy.

The presence of PCR inhibitors may cause false-negative or invalid results. The reference range for the Roche cobas HPV test is negative.

J Low Genit Tract Dis ;16 3 2. J Pathol ; 3. Lancet Oncol ; 4. Roche Cobas

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